Expressiona of c-Jun and collagens I and III in cultured human skin fibroblasts are affected by infrared ray radiation / 南方医科大学学报
Journal of Southern Medical University
;
(12): 163-169, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-232490
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of solar infrared ray (IR) radiation on the expressions of c-Jun and collagens I and III in cultured human skin fibroblasts (HSFs) and explore the molecular mechanism by which IR radiation causes aging of the skin.</p><p><b>METHODS</b>Primarily cultured HSFs exposed to IR radiation were examined for changes of the cell viability with MTT assay. The mRNA and protein expressions of c-Jun and collagens I and III was detected with real-time quantitative PCR and immunocytochemistry.</p><p><b>RESULTS</b>MTT assay showed that IR irradiation caused inhibition of cell proliferation compared with the control cells. The mRNA and protein expression of collagen I was decreased significantly by IR irradiation with the increase of the irradiation dose (P<0.01). HSFs irradiated by IR for 12 h showed a dose-dependent reduction of the expression of collagen type III mRNA and protein (P<0.05, P<0.01), but the expression increased dose-dependently in response to IR exposure for 24 h (P<0.05 or 0.01). IR irradiation enhanced the mRNA and protein expression of c-Jun in a dose-dependence manner (P<0.05 or 0.01).</p><p><b>CONCLUSIONS</b>IR irradiation can increase the expression of c-Jun, inhibit the expression of collagen I, and cause disturbance in collagen III expression in human skin fibroblasts, which may be one of the mechanism of IR radiation to initiate and promote skin photoaging.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Efeitos da Radiação
/
Pele
/
Raios Ultravioleta
/
RNA Mensageiro
/
Envelhecimento da Pele
/
Sobrevivência Celular
/
Células Cultivadas
/
Proteínas Proto-Oncogênicas c-jun
/
Biologia Celular
/
Colágeno Tipo I
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2016
Tipo de documento:
Artigo
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