Transforming growth factor β1 enhances the invasiveness of human glioma cell line via ERK/MAPK pathway / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of transforming growth factor β1 (TGF-β1) on the invasiveness of human glioma SF767 cell line in vitro and explore the possible molecular mechanism.</p><p><b>METHODS</b>Human glioma SF767 cell line treated with TGF-β1 for 12, 24, or 48 h were examined for the expressions of epithelial-mesenchymal transition- and ERK/MAPK pathway-associated proteins using Western blotting. MTT assay and Transwell assay were employed to assess the changes in the cell proliferation and invasiveness after TGF-β1 treatment, respectively.</p><p><b>RESULTS</b>Western blotting showed that TGF-β1 treatment up-regulated the expressions of vimentin, MMP-2, P-ERK, and Zeb-1 and down-regulated E-cadherin expression in SF767 cells. TGF-β1 treatment of the cells resulted in significantly shortened doubling time of cells and obviously increased cell invasiveness.</p><p><b>CONCLUSION</b>TGF-β1 induces epithelial-mesenchymal transition of SF767 cell line to increase its invasiveness possibly via ERK/MAPK pathway.</p>