Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAX- tG250FcGB.</p><p><b>METHODS</b>The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared.</p><p><b>RESULTS</b>The vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection.</p><p><b>CONCLUSION</b>Electroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.</p>