Effects of Toll-like receptors on indoleamine 2, 3-dioxygenase mRNA levels in human trophoblast HTR-8/SVneo cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1559-1564, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-232750
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of Toll-like receptors (TLRs) mRNA in human trophoblast HTR-8/SVneo cells and the changes in indoleamine 2,3-dioxygenase (IDO) mRNA expression in response to TLR ligand stimulation.</p><p><b>METHODS</b>The expressions of TLRs and IDO mRNA in human HTR-8/SVneo cells were tested by RT-PCR, and the changes in IDO mRNA levels after exposure to TLR3, TLR4, TLR7/8, and TLR9 ligands were quantitatively analyzed with real-time PCR.</p><p><b>RESULTS</b>IDO and TLR1-10 mRNAs were expressed in HTR-8/SVneo cells. As the cell culture time extended, IDO mRNA expression level tended to increase within 48 h. After stimulation with the TLR ligands, the expression of TLR-3 mRNA was down-regulated while the expression of TLR-4, 7, 8, and 9 mRNA up-regulated. Stimulation of the cells with poly(IC) lowered the expression of IDO mRNA while IFN-γ increased its expression.</p><p><b>CONCLUSIONS</b>The expression of IDO mRNA is associated with the nutrition of the maternal-fetal interface. Stimulation with the TLR ligands affects the expression of IDO and TLR mRNA expressions in the cells, which verifies the functional activity of TLRs and suggests a role of IDO in TLR pathway-dependent antiviral immunity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Trofoblastos
/
RNA Mensageiro
/
Linhagem Celular
/
Interferon gama
/
Poli I-C
/
Biologia Celular
/
Indolamina-Pirrol 2,3,-Dioxigenase
/
Receptores Toll-Like
/
Genética
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Artigo
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