Establishment of miniCTTA multiplex set with fluorescence-labeled primers / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To establish a miniCTTA multiplex set including short tandem repeat (STR) markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp.</p><p><b>METHODS</b>The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens.</p><p><b>RESULTS</b>The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit.</p><p><b>CONCLUSION</b>MiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.</p>