Your browser doesn't support javascript.
loading
Qualitative fingerprint and quantitative determination of caffeic acid in compound dandelion enema / 南方医科大学学报
Journal of Southern Medical University ; (12): 1503-1505, 2006.
Artigo em Chinês | WPRIM | ID: wpr-232848
ABSTRACT
<p><b>OBJECTIVE</b>To establish a qualitative and quantitative reversed-phase high-performance liquid chromatography (RP-HPLC) with fingerprinting technique for quality control of compound dandelion enema.</p><p><b>METHODS</b>HPLC was utilized for quality assessment of 10 batches of samples. RP-HPLC analysis was performed on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of acetonitrile (A) and potassium phosphate solution (B) (pH3.2) as the mobile phase in gradient mode. The concentrations of solvent A were 10%, 80% and 80% at 0, 38 and 40 min, respectively. The column temperature was set at 35 degrees C, the flow rate at 0.7 ml/min and the detection wavelength at 254 nm.</p><p><b>RESULTS</b>HPLC fingerprinting was established from the 10 batches, and the data showed 23 characteristic peaks in the compound dandelion enema for use as index peaks for qualitative identification. Comparison of the retention time and the on-line UV spectra of the samples with the chemical standards identified peaks 3, 4 and 8 as protocatechualdehyde, caffeic acid and ferulic acid, respectively. The contents of caffeic acid in the compound dandelion enema ranged between 63.7 and 136.8 microg/ml.</p><p><b>CONCLUSION</b>High specific chromatographic fingerprinting and quantitative measurement of caffeic acid allows rigorous quality control of compound dandelion enema.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Padrões de Referência / Ácidos Cafeicos / Medicamentos de Ervas Chinesas / Química / Reprodutibilidade dos Testes / Cromatografia Líquida de Alta Pressão / Taraxacum / Métodos Tipo de estudo: Estudo prognóstico / Pesquisa qualitativa Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Padrões de Referência / Ácidos Cafeicos / Medicamentos de Ervas Chinesas / Química / Reprodutibilidade dos Testes / Cromatografia Líquida de Alta Pressão / Taraxacum / Métodos Tipo de estudo: Estudo prognóstico / Pesquisa qualitativa Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo