Anti-MDR tumor mechanism of CIP-36, a podophyllotoxin derivative / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1193-1198, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-233013
ABSTRACT
This study is to investigate the antitumor activity of CIP-36 on multidrug resistant human oral squamous carcinoma cell line (KBV200 cells) in vitro and the possible anticancer mechanisms. MTT assay, Hoechst fluorescein stain, RT-PCR and immunohistochemistry were carried out on KBV200 and KB cells. The growth of many tumor cells was obviously inhibited by CIP-36, especially the multidrug resistant cells KBV200. Obvious apoptosis could be observed in the Hoechst 33342 staining experiments. The results of RT-PCR showed that the levels of p53, p21, caspase-3 and bax mRNA increased, and meanwhile the expression of mdr-1 and bcl-2 mRNA decreased in a dose-dependent manner. The data were significantly different from that of vehicle. The expression of P-gp significantly decreased with the increasing dosage of CIP-36 examined by immunohistochemistry. It can be concluded that CIP-36 could change resistance-related genes and proteins to overcome multidrug resistance in the KBV200 cell line.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Podofilotoxina
/
RNA Mensageiro
/
Neoplasias Bucais
/
Células KB
/
Carcinoma de Células Escamosas
/
Proteína Supressora de Tumor p53
/
Proteínas Proto-Oncogênicas p21(ras)
/
Apoptose
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2011
Tipo de documento:
Artigo
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