Development of fluorescence imaging based assay for screening compounds with anti-migration activity / 药学学报
Acta Pharmaceutica Sinica
;
(12): 793-797, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-233054
ABSTRACT
In the present study, A fluorescent imaging-based high-throughput screening method was developed for identifying anti-migratory compounds with 96-well Transwell plates. The correlation, precision and stability of this method were examined and the incubation time of dye Hoechst 33342 in addition to migration time was optimized. In addition, The inhibitory activity of anti-cancer drug paclitaxel on tumor cell migration was assayed and an IC50 value of 0.717 micromol x L(-1) was obtained. Using this method, 24 components from Rhizoma Alismatis were screened and one component with anti-migration activity was found. These results show that the new proposed method with good precision, stability and linear range has the potential to assay the inhibitory activity of anticancer compounds.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plantas Medicinais
/
Ensaios de Seleção de Medicamentos Antitumorais
/
Medicamentos de Ervas Chinesas
/
Movimento Celular
/
Química
/
Paclitaxel
/
Concentração Inibidora 50
/
Rizoma
/
Alisma
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2011
Tipo de documento:
Artigo
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