Regulatory effect of 11 kDa protein of parvovirus B19 on NF-kappaB pathway in Hela cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 965-973, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-233183
ABSTRACT
The 11 kDa protein, a small nonstructural protein of parvovirus B19, may play important roles in viral replication cycle. To investigate the effect of 11 kDa protein on the NF-kappaB signaling pathway, we first prepared the poly-antiserum using GST-11 kDa fusion protein purified via prokaryotic expression system, and demonstrated that the 11 kDa protein mainly localized in cytoplasm when expressed in Hela cells. Meanwhile, luciferase activity assay and Western blotting assay showed that 11 kDa up-regulated the transcriptional activity of NF-kappaB and induced the degradation of IkappaB-alpha in Hela cells. Moreover, the 11 kDa protein activated the IL6 promoter, which is probably through the NF-kappaB pathway. Taken together, these results suggested that 11 kDa protein may contribute to activating inflammatory factors through participating in the cell signaling pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células HeLa
/
Transdução de Sinais
/
Ativação Transcricional
/
NF-kappa B
/
Regiões Promotoras Genéticas
/
Parvovirus B19 Humano
/
Proteínas não Estruturais Virais
/
Proteínas I-kappa B
/
Inibidor de NF-kappaB alfa
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2013
Tipo de documento:
Artigo
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