Cell-ELA-based determination of binding affinity of DNA aptamer against U87-EGFRvIII cell / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 664-671, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-233211
ABSTRACT
A15, a DNA aptamer with binding specificity for U87 glioma cells stably overexpressing the epidermal growth factor receptor variant III (U87-EGFRvIII), was generated by cell systematic evolution of ligands by exponential enrichment (cell-SELEX) using a random nucleotide library. Subsequently, we established a cell enzyme-linked assay (cell-ELA) to detect the affinity of A15 compared to an EGFR antibody. We used A15 as a detection probe and cultured U87-EGFRvIII cells as targets. Our data indicate that the equilibrium dissociation constants (K(d)) for A15 were below 100 nmol/L and had similar affinity compared to an EGFR antibody for U87-EGFRvIII. We demonstrated that the cell-ELA was a useful method to determine the equilibrium dissociation constants (K(d)) of aptamers generated by cell-SELEX.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Ligação Proteica
/
Neoplasias Encefálicas
/
Linhagem Celular Tumoral
/
Aptâmeros de Nucleotídeos
/
Técnica de Seleção de Aptâmeros
/
Receptores ErbB
/
Genética
/
Glioma
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2013
Tipo de documento:
Artigo
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