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Expression, crystallization and crystallographic study of the 1st IgV domain of human CD96 / 生物工程学报
Chinese Journal of Biotechnology ; (12): 657-663, 2013.
Artigo em Chinês | WPRIM | ID: wpr-233212
ABSTRACT
CD96 (Tactile) is an adhesion receptor expressed mainly on activated T cells, NK cells. As a family member of the immunoglobulin-like cell receptor, CD96 consists of three immunoglobulin-like domains (V1, V2/C and C) in the extracellular region. Recent studies have shown that the 1st IgV domain of CD96 (CD96V1) plays an essential role in cell adhesion and NK cell-mediated killing. In this study, the 1st IgV domain of human CD96 (hCD96V1) was cloned and expressed in Escherichia coli (BL21). The soluble protein was obtained by refolding of the hCD96V1 inclusion bodies. From analytical ultracentrifugation, we could predict that CD96 V1 maily exists as dimer with approximate molecular weight of 26.9 kDa. The protein was then successfully crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to 1.9 angstrom resolution and belonged to space group P21, with unit-cell parameters a = 35.1, b = 69.5, c = 49.6A, alpha=gamma=90 degrees, beta=105.4 degrees.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Recombinantes / Região Variável de Imunoglobulina / Antígenos CD / Estrutura Terciária de Proteína / Cristalização / Cristalografia / Escherichia coli / Genética / Metabolismo / Métodos Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Recombinantes / Região Variável de Imunoglobulina / Antígenos CD / Estrutura Terciária de Proteína / Cristalização / Cristalografia / Escherichia coli / Genética / Metabolismo / Métodos Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2013 Tipo de documento: Artigo