Quantitative analysis of γ-oryzanol content in cold pressed rice bran oil by TLC-image analysis method

ABSTRACT

<p><b>OBJECTIVE</b>To develop and validate an image analysis method for quantitative analysis of γ-oryzanol in cold pressed rice bran oil.</p><p><b>METHODS</b>TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test.</p><p><b>RESULTS</b>Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol.</p><p><b>CONCLUSIONS</b>The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.</p>