Detection of EML4-ALK fusion gene in non-small cell lung cancer and its clinicopathologic correlation / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 252-256, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-233478
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequency of EML4-ALK fusion gene in non-small-cell lung cancer (NSCLC) patients, and its correlation with clinicopathologic features.</p><p><b>METHODS</b>Real-time PCR was used to detect the presence of EML4-ALK fusion gene in 268 cases of NSCLCs using paraffin-embedded tissue samples(among which 164 samples were re-validated by Sanger sequencing). Related clinicopathological correlation was analyzed.</p><p><b>RESULTS</b>EML4-ALK fusion gene was found in 4.1% (11/268) of the cases. One hundred and sixty four samples were verified by Sanger sequencing, and the overall coincidence of the results of two methods (Sanger sequencing and Real-time PCR) was 100%. Female patients (5.9%, 5/85), ≤ 60 years of age (4.3%, 6/140), non-smokers (6.8%, 8/118) and adenocarcinomas (7.6%, 10/132) had a higher mutation rate than that in male patients (3.3%, 6/183), > 60 years of age (4.0%, 5/124), smokers (1.6%, 2/132) and squamous cell carcinomas (1.3%, 1/79), although no statistical significance in age (P = 0.918), gender (P = 0.503), smoking history (P = 0.092) and histological type (P = 0.094).</p><p><b>CONCLUSIONS</b>Chinese NSCLC patients have a 4.1% detection rate of EML4-ALK fusion gene in the tumor tissues. Female, non-smoker and adenocarcinoma histological subtype tend to be associated with a higher rate of EML4-ALK gene fusion.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Cirurgia Geral
/
Adenocarcinoma
/
Fumar
/
Proteínas de Fusão Oncogênica
/
Fatores Sexuais
/
Carcinoma Pulmonar de Células não Pequenas
/
Genética
/
Neoplasias Pulmonares
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2013
Tipo de documento:
Artigo
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