Killing activity in DC and CIK co-culture against hepatocarcinoma cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 543-546, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-233549
ABSTRACT
This study was aimed to investigate the proliferation activities and phenotype changes of DC, CIK and DC-CIK, and their cytotoxicity against hepatocarcinoma cells in co-culture of DC with CIK. Peripheral blood mononuclear cells (PBMNC) were isolated from healthy adult donors. After incubation of PBMNC for 2 hours, DCs were induced from adherent cells by some cytokines and CIKs were generated from non-adherent cells. Mature DCs were harvested after incubation for 9 days, and then were co-cultured with CIK at ratio of 15 for 3 days. The cytotoxicity activity against SMMC-7721 hepatocellular carcinoma cell line was detected by MTT assay. The results showed that CIK cells were able to lyse SMMC-7721 hepatocellular carcinoma cells at low ratios of effector to target. This effect was significantly enhanced by co-culture with DCs. It is concluded that CIK cells have high lytic activity against 7721 hepatocellular carcinoma cell line, which can be enhanced by co-culture with DC. DC-CIK cells are highly effective immune cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Células Dendríticas
/
Células Matadoras Ativadas por Linfocina
/
Células Cultivadas
/
Carcinoma Hepatocelular
/
Técnicas de Cocultura
/
Biologia Celular
/
Citotoxicidade Imunológica
/
Alergia e Imunologia
/
Neoplasias Hepáticas
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo
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