Recombinant adenovirus-mediated expression of 1.2-copy hepatitis B virus DNA in three hepatocytes / 南方医科大学学报
Journal of Southern Medical University
; (12): 738-740, 2009.
Article
em Zh
| WPRIM
| ID: wpr-233695
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study hepatitis B virus (HBV) expression in 3 hepatocytes infected with recombinant adenovirus containing 1.2-copy HBV DNA.a</p><p><b>METHODS</b>A chicken hepatoma cell line and two human hepatocytes were infected by the recombinant adenovirus containing 1.2-copy HBV DNA at 25 pfu/cell. HBV-specific mRNA was detected by RT-PCR 3 days after the infection, and HBsAg and HBeAg were detected by ELISA and HBV DNA by real-time PCR daily after the infection.</p><p><b>RESULTS</b>HBV mRNA expression was detected in all the 3 cells after recombinant adenovirus infection, and the quantities of HBV DNA and HBV antigens in the culture supernatant increased with the passage of time.a</p><p><b>CONCLUSION</b>Infection with the recombinant adenovirus containing 1.2-copy HBV DNA can mediate HBV infection in the 3 cells in vitro.</p>
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Índice:
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Assunto principal:
Virologia
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DNA Recombinante
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DNA Viral
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Expressão Gênica
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Adenoviridae
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Vírus da Hepatite B
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Meios de Cultivo Condicionados
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Hepatócitos
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Linhagem Celular Tumoral
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Article