Effects of Ciwujia in inducing marrow stromal cell differentiation into neuron-like cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 487-490, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-233756
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of the traditional Chinese herbal drug Ciwujia in inducing the differentiation of marrow stromal cells (MSCs) into neuron-like cells.</p><p><b>METHODS</b>Rat MSCs isolated from the whole bone marrow were amplified by adherent culture in vitro and induced to differentiate into neuron-like cells using serum-free DMEM/F12 containing Ciwujia. The protein and mRNA expressions of nestin, beta-Tubulin III and glial fibrillary acidic protein (GFAP) in the differentiated cells were detected by indirect immunofluorescence method, Western blotting and RT-PCR.</p><p><b>RESULTS</b>The third-passage MSCs showed positive expression rates for CD44 and CD54 beyond 90% with decreased CD14 expression rate to 2.37%. Induction by Ciwujia of the MSCs resulted in cell body shrinkage and protrusion of the cell processes resembling those of neurons. The differentiated cells were positive for nestin and beta-Tubulin III expression and negative for GFAP as shown by immunofluorescence assay, Western blotting and RT-PCR.</p><p><b>CONCLUSION</b>Ciwujia can induce the differentiation of rat MSCs into neuron-like cells in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Tubulina (Proteína)
/
Células da Medula Óssea
/
Medicamentos de Ervas Chinesas
/
Extratos Vegetais
/
Diferenciação Celular
/
Células Cultivadas
/
Células Estromais
/
Ratos Wistar
/
Eleutherococcus
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo
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