Your browser doesn't support javascript.
loading
Expression of ERK5 in multidrug-resistant hepatocellular carcinoma cell line / 南方医科大学学报
Journal of Southern Medical University ; (12): 483-486, 2009.
Artigo em Chinês | WPRIM | ID: wpr-233757
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of extracellular-signal regulated protein kinase 5 (ERK5) in multidrug-resistant hepatocellular carcinoma cell lines hepG2/ADM and BEL-7402/5-FU and in the parental cell lines (hepG2 and BEL-7402), and study the role of ERK5 in multidrug resistance of hepatocellular carcinoma.</p><p><b>METHODS</b>MTT assay was used to determine the multidrug resistance (MDR) of hepG2/ADM and BEL-7402/5-FU cells. Real-time PCR and Western blotting were used to detect the expression of ERK5 at mRNA and protein levels in the 4 cell lines.</p><p><b>RESULTS</b>The resistance indices to ADM, 5-FU, and CDDP was 12.34, 5.74, and 3.81 in hepG2/ADM cells, and the resistance indices to 5-FU, VCR, OHP, MTX, and ADM was 15.32, 10.08, 5. 85, 6.74 and 3.26 in BEL-7402 cells, respectively. Compared with those in hepG2 and BEL-7402 cells, the ERK5 gene was up-regulated at both mRNA and protein levels in HepG2/ADM cells, but down-regulated at the mRNA level and up-regulated at the protein level in BEL-7402/5-FU cells.</p><p><b>CONCLUSIONS</b>ERK5 is related to multidrug resistance in HepG2/ADM and BEL-7402/5-FU cells, and may provide new clues for reversing multidrug resistance of hepatocellular carcinoma cells.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / RNA Mensageiro / Células Tumorais Cultivadas / Doxorrubicina / Carcinoma Hepatocelular / Resistência a Múltiplos Medicamentos / Resistencia a Medicamentos Antineoplásicos / Proteína Quinase 7 Ativada por Mitógeno / Fluoruracila Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2009 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / RNA Mensageiro / Células Tumorais Cultivadas / Doxorrubicina / Carcinoma Hepatocelular / Resistência a Múltiplos Medicamentos / Resistencia a Medicamentos Antineoplásicos / Proteína Quinase 7 Ativada por Mitógeno / Fluoruracila Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2009 Tipo de documento: Artigo