Identification of the hemolytic transfusion reaction caused by lewis antibody using serological and molecular biological methods / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1192-1195, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-234269
ABSTRACT
To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le (a-b-) phenotype cells, patient phenotype was identified by using anti-Le(a) and anti-Le(b) blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly. The results showed that both IgM anti-Le(a) and anti-Le(b) antibodies were detected in patient's serum. Red cells was typed as Le (a-b-) phenotype and the FUT3 genotype was homozygote for non-functional le(59, 508) alleles. In conclusion, anti-Le(b) antibody can result in hemolytic transfusion reaction, FUT3 gene is homozygous for le(59, 508) allele resulting in Le (a-b-) phenotype.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sorologia
/
Tipagem e Reações Cruzadas Sanguíneas
/
Alergia e Imunologia
/
Reação Transfusional
/
Fucosiltransferases
/
Genética
/
Genótipo
/
Doenças Hematológicas
/
Antígenos do Grupo Sanguíneo de Lewis
/
Anticorpos
Tipo de estudo:
Estudo prognóstico
Limite:
Adulto
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2008
Tipo de documento:
Artigo
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