Establishment of allele specific primer polymerase chain reaction sequence-based typing and investigation of the polymorphism in exon 3 of HLA-DRB1 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 639-643, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-234347
ABSTRACT
<p><b>OBJECTIVE</b>To establish the allele specific primer polymerase chain reaction sequence-based typing (PCR-SBT) and investigate the polymorphism of exon 3 of human leukocyte antigen( HLA)-DRB1.</p><p><b>METHODS</b>The fragment containing exons 2 and 3 of HLA-DRB1 gene was amplified by group specific primers. The amplified products were digested by restriction enzymes and directly sequenced in both directions. The genotype was assigned by using Assign 3.5 SBT software.</p><p><b>RESULTS</b>The exon 3 sequences of HLA-DRB1*0809 and HLA-DRB1*120201 were identified for the first time. There were 27 polymorphic sites in exon 3 among the twenty-five HLA-DRB1 alleles, which was 9.56% of all nucleotides of exon 3. The method could discriminate the HLA-DRB1*140101/1454 ambiguous samples, and the HLA-DRB1*140101 was identified in the Chinese population.</p><p><b>CONCLUSION</b>The PCR-SBT method for exon 3 of HLA-DRB1 from the present study was reliable and there were polymorphisms in exon 3 of HLA-DRB1.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Polimorfismo Genético
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Antígenos HLA-DR
/
Reação em Cadeia da Polimerase
/
Éxons
/
Primers do DNA
/
Evolução Molecular
/
Alelos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2010
Tipo de documento:
Artigo
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