Effect of bone morphogenetic protein 4 on glioma stem cell proliferation and apoptosis in vitro / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 484-488, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-234377
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of bone morphogenetic protein 4 (BMP4) on the proliferation and apoptosis in glioma stem cells.</p><p><b>METHODS</b>Stem cells were isolated from a human glioma cell line U87 by using vincristine and characterized by immunofluorescence assay. Proliferation and apoptosis were determined by soft agar colony assay and flow cytometry; Cyclin D1, Bcl-2 and Bax were detected by Western blot analysis.</p><p><b>RESULTS</b>BMP4 inhibited cell proliferation and promoted apoptosis in U87 glioma stem cells. Moreover, Bcl-2 and Cyclin D1 expression were decreased by BMP4, while Bax level was elevated.</p><p><b>CONCLUSION</b>BMP4 can inhibit U87 glioma stem cells proliferation through downregulating Cyclin D1 level, and promote apoptosis through induction of Bax expression and inhibition of Bcl-2 level. It suggests that BMP4 plays an important role in human glioma stem cell biology.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco Neoplásicas
/
Regulação Neoplásica da Expressão Gênica
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Ciclina D1
/
Biologia Celular
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Proteína Morfogenética Óssea 4
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2010
Tipo de documento:
Artigo
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