Investigation on bioactive protective function of amino acids to insulin by molecular simulation / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 822-826, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-234503
ABSTRACT
Heat sensitive protein medicines are increasingly exhibiting their critical importance on treatment of various diseases at present. But their popularization and application meet a great challenge because of their heat instability. In the present study, insulin was taken as a heat sensitive protein medicine and amino acid as bio-protective agent in order to investigate if these amino acids can protect the insulin from losing its bioactivity due to desiccation. The experiment was performed by using replica exchange molecular simulation (REMD) method and Gromacs software with Gromos96 (53a6) force field. The REMD results indicated that these amino acids could protect the bioactive structure of insulin during desiccation. The configurations of the protected insulin were preserved very well. Those results proved that amino acid is a kind of good bioactive protective agent for the heat sensitive protein medicines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Software
/
Química
/
Dessecação
/
Estabilidade de Medicamentos
/
Simulação de Dinâmica Molecular
/
Aminoácidos
/
Insulina
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2014
Tipo de documento:
Artigo
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