Variation of TFAR-19 protein expression in the thymus of emotional stress mice and the regulatory effect of Modified Xiaoyao Pill / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 626-628, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-234724
ABSTRACT
<p><b>OBJECTIVE</b>To study the variation of TFAR-19 protein expression and Annexin-V apoptosis protein proportion in the mice thymus cell apoptosis procedure induced by water platform environment or electric emotional stress and the regulatory effect of Modified Xiaoyao Pill (MXP).</p><p><b>METHODS</b>The mouse emotional stress model was established by water platform equipment or electrical stimulation. The serum glucocorticoid was detected by radioimmunoassay, the TFAR-19 protein was detected by flow cytometry analysis, and Annexin-V apoptosis protein proportion was calculated by immunofluorescence technique.</p><p><b>RESULTS</b>In the groups of mouse stressed by water platform environment, the level of serum glucocorticoid, the TFAR-19 protein expression and the Annexin-V apoptosis protein proportion increased in the thymus cell along with the stress time prolonging (P<0.05 or P <0.01). The serum glucocorticoid level in mice treated with MXP was lower than that in the untreated group (P <0.05). In the groups of emotional stressed mouse established by electrical stimulation, the above-mentioned variations also revealed. All these variations could be alleviated with MXP (P<0.05).</p><p><b>CONCLUSION</b>The water platform environment stress is a chronic continuous stress and electrical stimulation is an acute smooth stress, both of them could damage thymus function through neuro-endocrineo-immune network, but different in duration for causing severe injury. Chinese medicine MXP can alleviate the damage of thymus induced by either of them to certain degree.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Estresse Psicológico
/
Timo
/
Sangue
/
Medicamentos de Ervas Chinesas
/
Anexina A5
/
Biologia Celular
/
Proteínas Reguladoras de Apoptose
/
Glucocorticoides
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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