Effect of shenkang injection on hypertrophy and expressions of p21 and p27 in glomerular mesangial cells of rats cultured in high glucose / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 68-71, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-234805
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Shenkang Injection (SI) on hypertrophy and expressions of p21 and p27 in glomerular mesangial cells of rats cultured in high glucose fluid.</p><p><b>METHODS</b>Cultured rat glomerular mesangial cells were divided into 6 groups the normal control group, mannitol-treated group, high glucose-treated group, high, middle and low dose SI treated groups. The synthesis of cell protein and DNA were determined with incorporation of 3H-TdR and 3H-Leu. The changes of p21 mRNA and expressions of p21 and p27 protein were determined by reverse transcriptase polymerase chain reaction and Western blot.</p><p><b>RESULTS</b>High glucose caused incorporation of 3H-TdR reduced, incorporation of 3H-Leu increased. SI could decrease incorporation of 3H-Leu, increase incorporation of 3H-TdR. Compared with the high glucose-treated group, SI could inhibit the overexpression of p2l mRNA and protein,p27 protein induced by high glucose.</p><p><b>CONCLUSION</b>SI could inhibit hypertrophy of glomerular mesangial cells cultured in high glucose partly through inhibiting overexpressions of p2l mRNA, p21 and p27 protein.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Células Cultivadas
/
Meios de Cultura
/
Biologia Celular
/
Crescimento Celular
/
Células Mesangiais
/
Inibidor de Quinase Dependente de Ciclina p21
/
Inibidor de Quinase Dependente de Ciclina p27
/
Glucose
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2006
Tipo de documento:
Artigo
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