Effect of ingredients of Astragalus-Salvia compound on vascular endothelial cell in placenta and vascular endothelial growth factor mRNA expression in trophocyte in pregnant rats with inhibited nitric oxide synthesis / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 516-519, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-234835
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanisms of effective ingredients of Astragalus-Salvia compound (ASC) in increasing placental blood supply, to provide thought for establishing an effective prevention and treatment of insufficient placental blood supply caused complications of pregnancy.</p><p><b>METHODS</b>The effective ingredients of ASC was isolated and abstracted and the rat model of inhibited nitric oxide synthesis was established by using L-Arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor. The mRNA expression of vascular endothelial growth factor (VEGF) in placental trophocyte and primordial micrangial endothelial cell was determined by using in situ hybridization at 12th day of pregnancy.</p><p><b>RESULTS</b>The VEGF mRNA expression was significantly lower in nitric oxide synthesis inhibited model rats than that in rats non-modeled, or in model rats treated by ASC abstracts or nitroglycerin (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>Effective ingredients of ASC may improve VEGF mRNA expression in placental trophocyte and vascular endothelial cell in early pregnancy, and thus be favorable for placental vascular net formation, trophocyte infiltration and mother-placental circulation constitution for increasing placental blood supply to prevent and treat the insufficient placental blood supply caused diseases such as pregnancy-induced hypertension syndrome.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Placenta
/
Trofoblastos
/
RNA Mensageiro
/
Medicamentos de Ervas Chinesas
/
Química
/
Ratos Sprague-Dawley
/
Astragalus propinquus
/
Salvia miltiorrhiza
/
Biologia Celular
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Gravidez
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo
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