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Effect of fuzheng yiliu granule on H22 tumor cell apoptosis, p53 and caspase-3 gene expression in mice / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 338-341, 2005.
Artigo em Chinês | WPRIM | ID: wpr-234879
ABSTRACT
<p><b>OBJECTIVE</b>To explore the inhibitory effect of Fuzheng Yiliu Granule (FYG) on growth and apoptosis of H22 tumor cell, and expressions of p53 and Caspase-3 gene.</p><p><b>METHODS</b>The tumor inhibitory rate of FYG on H22 tumor cell line was observed in vivo, cell apoptosis rate and cell cycle were determined by flow cytometry (FCM), and expressions of wild type p53 and Capase-3 mRNA were determined by RT-PCR.</p><p><b>RESULTS</b>FYG could inhibit the growth of H22 tumor cell at the dose of 12g/kg, 24g/kg, the maximal inhibitory rate up to 51.24% (P < 0.01). By FCM, it was shown that FYG could significantly enhance apoptosis rate of cell line H22, with the peak reached 15.84% (P < 0.01), and cause the tumor cell cycle being blocked at G0/G1 phase, with decrease of cells in S phase. RT-PCR illustrated that FYG could significantly up-regulate the level of p53 and Caspase-3 mRNA expression.</p><p><b>CONCLUSION</b>FYG can significantly inhibit the growth of tumor cell in mice, its anti-tumor mechanisms may relate to the cell apoptosis induction, cell cycle regulation and wild type p53 and capase-3 gene expression enhancing.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Medicamentos de Ervas Chinesas / Distribuição Aleatória / Proteína Supressora de Tumor p53 / Apoptose / Carcinoma Hepatocelular / Caspases / Linhagem Celular Tumoral / Proliferação de Células Limite: Animais Idioma: Chinês Revista: Chinese Journal of Integrated Traditional and Western Medicine Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Medicamentos de Ervas Chinesas / Distribuição Aleatória / Proteína Supressora de Tumor p53 / Apoptose / Carcinoma Hepatocelular / Caspases / Linhagem Celular Tumoral / Proliferação de Células Limite: Animais Idioma: Chinês Revista: Chinese Journal of Integrated Traditional and Western Medicine Ano de publicação: 2005 Tipo de documento: Artigo