Establishment of a green fluorescent protein-labeled mouse model of adenoid cystic carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 424-428, 2011.
Article
em Zh
| WPRIM
| ID: wpr-235028
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a novel nude mice model which can be visualized in real time and detected in a continuous and dynamic way for the development and metastasis of adenoid cystic carcinoma.</p><p><b>METHODS</b>Human adenoid cystic carcinoma cells, ACCM cell line, were infected with retroviral vector of pLEGFP-N1 and then screened for a single colony of ACCM-GFP cells. Cell proliferation and morphological analysis were conducted for ACCM and ACCM-GFP cells. Nude mice lingual carcinoma model was set up with ACCM-GFP cells injection and real time observation with fluorescence imaging on ACCM-GFP tumors was performed subsequently. Histological assay was analyzed for ACCM and ACCM-GFP tumors as well.</p><p><b>RESULTS</b>ACCM-GFP cells were able to express GFP stably in the long term. ACCM and ACCM-GFP cells showed no significant difference in cell proliferation and morphology, and no significant difference of histological characteristics in vivo could be found between ACCM and ACCM-GFP tumors. Tumor development could be monitored in real time with fluorescence imaging system in vivo.</p><p><b>CONCLUSION</b>GFP-expressing ACCM tumor model can be applied to detect and observe its development in the long term in a noninvasive, real time and dynamic way. It is also a kind of ideal in vivo mouse model for adenoid cystic carcinoma research.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Carcinoma Adenoide Cístico
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Linhagem Celular Tumoral
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Proteínas de Fluorescência Verde
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Proliferação de Células
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Modelos Animais de Doenças
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Camundongos Endogâmicos BALB C
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Camundongos Nus
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2011
Tipo de documento:
Article