Expression of glucose transporter-1 and insulin receptor alpha1 in osteoblast obtained from diabetic rats' mandibles / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression of glucose transporter (GLUT)-1 and insulin receptor (IR) alpha1 in osteoblast obtained from diabetic rats' mandibles.</p><p><b>METHODS</b>The expression of GLUT-1 and IR alpha1 of diabetic and control groups were measured by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry stain.</p><p><b>RESULTS</b>The mRNA expressions of GLUT-1 and IR alpha1 of diabetic group were significantly higher than control group (P<0.05). The protein expressions of GLUT-1 and IR alpha1 were similar to the mRNA expressions.</p><p><b>CONCLUSION</b>Osteoblasts obtained from diabetic rats' mandibles keep the adaptation changes to hyperglycemia and hypoinsulinemia, which may contribute to their dysfunction.</p>