Activation of Rho-kinase pathway is involved in angiotensin II-induced contraction of human airway smooth muscle cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1416-1420, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-235112
ABSTRACT
<p><b>OBJECTIVE</b>To investigate of the regulatory effect of Rho-kinase pathway activation on angiotensin II (Ang II)-induced contraction of human airway smooth muscle cells (HASMCs) in vitro.</p><p><b>METHODS</b>Cultured primary HASMCs were divided into control group, AngII group, AngII + irbesartan group and AngII + Y-27632 group with corresponding treatment. AngII-induced contraction of HASMCs was evaluated using collagen gel lattices and observed morphologically using immunofluorescence assay. Western Blotting was significantly performed to examine the protein expression of Rho-kinase signal pathway.</p><p><b>RESULTS</b>AngII-induced HASMC contraction was inhibited by treatments with irbesartan and Y-27632 as shown by gel contraction assay (P<0.001). Y-27632 treatment produced a stronger inhibitory effect than irbesartan on the expression of phosphorylated moesin, a substrate of Rho kinase (P<0.05).</p><p><b>CONCLUSION</b>AngII induces the contraction of HASMCs partially as a result of activation of Rho-kinase pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Piridinas
/
Asma
/
Tetrazóis
/
Compostos de Bifenilo
/
Brônquios
/
Angiotensina II
/
Transdução de Sinais
/
Biologia Celular
/
Quinases Associadas a rho
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
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