Identification of the IgE-binding epitopes in main dust mite allergen Der p 1 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1183-1186, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-235168
ABSTRACT
<p><b>OBJECTIVE</b>To identify the IgE-binding epitopes in the allergen Der p 1 of main house dust mites, which can be recognized by the specific IgE in the sera from allergic individuals, and obtain a hypoallergen derived from the T-B epitope fused peptide for potential use in specific immunotherapy (SIT).</p><p><b>METHODS</b>Thirty-one peptides containing 15 amino acids each, which covered the full 222 amino acids of Der p 1 protein sequence, were synthesized on the cellulous membrane by solid-phase peptide (SPOTs) synthesis, with 8 overlapping amino acids between every two neighboring peptides. The membrane bearing the spots of the synthesized peptides were incubated with the allergic serum pools consisting of the sera from 5 allergic individuals. The membrane was then probed with HRP-conjugated anti-human IgE, followed by enhanced chemiluminescence (ECL) for visualization and gray scale analysis of the positive peptide spots.</p><p><b>RESULTS</b>Three strong IgE-binding epitopes were identified in the amino acid sequence of Der p 1 molecule, namely Ep1 (amino acids 85-99), Ep2 (amino acids 106-120) and Ep3 (amino acids 190-204).</p><p><b>CONCLUSION</b>The 3 IgE-binding epitopes (B cell epitopes) identified in Der p 1 confirm the presence of linear epitopes in Der p 1, suggesting the possibility of constructing T/B epitope-fused hypoallergens.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sítios de Ligação de Anticorpos
/
Imunoglobulina E
/
Cisteína Endopeptidases
/
Dados de Sequência Molecular
/
Sequência de Aminoácidos
/
Linfocinas
/
Antígenos de Dermatophagoides
/
Alergia e Imunologia
/
Proteínas de Artrópodes
/
Ácaros
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
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