Study on separation and purification of total flavones from Smilax china by macroporous absorption resin / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the technological parameters of the purification process of total flavones from Smilax china with macroporous absorption resin.</p><p><b>METHOD</b>The technical process for purification of total flavones with the optimum macroporous absorption resin was screened by yield of total flavones product.</p><p><b>RESULT</b>The D140 macroporous absorption resin had the best separating efficiency when the flavones content in the liquid was 0.5 g x mL(-1) equivalent to raw material, the volume of drug 18 BV (resin bad volume) with the adsorption-power 2 BV x h(-1), and the volume of 60% (mL x mL(-1)) ethanol as eluant 5-10 BV (resin bad volume) with desorption-power 1 BV x h(-1). The obtained flavones product has total flavones recovery rate of 84.72%.</p><p><b>CONCLUSION</b>The treatment of regenerated resin is easy, this method is advisable.</p>