A new method to obtain molecular marker of sequence-tagged site (STS) of Panax ginseng and P. quinquefolius / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1012-1015, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-235276
ABSTRACT
<p><b>OBJECTIVE</b>Searching a new molecular method to authenticate Panax ginseng and P. quenquefolium.</p><p><b>METHOD</b>Single primers based on rDNA sequences of Panax species were designed to obtain polymorphic bands of P. ginseng and P. quinquefolius and then sequenced. Four PCR primers (two forword and two reverse primers) specific to P. ginseng and P. quinquefolius were designed.</p><p><b>RESULT</b>Primer Pg-6F, Pg-479R only amplified 474 bp band for P. ginseng and primer Pq-442F, Pq-658R only amplified 217 bp band for P. quinquefolius. It is indicated that the four primers could serve as specific STS primers for Panax species.</p><p><b>CONCLUSION</b>A new way to obtain STS primers of Panax species was established. This method is more quick and efficient than SCAR-PCR method and can serve as a model to obtain molecular markers for other Chinese material medica.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Polimorfismo Genético
/
Especificidade da Espécie
/
DNA Ribossômico
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Marcadores Genéticos
/
Sitios de Sequências Rotuladas
/
Classificação
/
Análise de Sequência de DNA
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS