Determination of benzopyrene in red clover extract by high performance liquid chromatography with spectrofluorescence detector / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 935-937, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-235287
ABSTRACT
<p><b>OBJECTIVE</b>To establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract.</p><p><b>METHOD</b>Analysis was performed on Hypersil C18 column (4. 6 mm x 200 mm, 5 microm) with acetonitrile and water (70 30) as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>The linear range was 0.93-37.2 ng x mL(-1), and r = 0.9992 (n = 5). The average recovery of benzopyrene was 85.2% (n = 9).</p><p><b>CONCLUSION</b>The method was convenient and accurate with good recovery.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Benzo(a)pireno
/
Medicamentos de Ervas Chinesas
/
Química
/
Reprodutibilidade dos Testes
/
Cromatografia Líquida de Alta Pressão
/
Trifolium
/
Fluorescência
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2007
Tipo de documento:
Artigo
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