Studies on callus culture of Akebia trifoliata / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 899-901, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-235299
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inductive factors of effect on Akebia trifoliata and establish culture method for A. trifoliata callus.</p><p><b>METHOD</b>To study the possible effective factors of culture condition by comparing with different explantation, nutrient medium, pH, temperature, illumination, growth substance of plant and its ratio.</p><p><b>RESULT</b>The inductivity of leaves was the highest about 87.5%, followed with the stem section and leafstalk; The inductivity of nutrient medium such as MS, B5 callus was higher than the ones like H, SH and the White callus amended one; It was found that low-grade Phvalue benefits the growth of callus. The experiment result showed that different pH showed little difference in quality. The best condition of culture was 25 degrees C in temperature.</p><p><b>CONCLUSION</b>The best culture condition for callus was the leaves as explantation. The A. trifoliata callus culture's best inductive condition was MS +2, 4-D 4.0 mg x L(-1) + NAA 1.0 mg x L(-1) + KT 1.0 mg x L(-1) (pH 5.8), cultural temperature was 25 degrees C, cultivation was dark.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plantas Medicinais
/
Temperatura
/
Química
/
Caules de Planta
/
Folhas de Planta
/
Magnoliopsida
/
Meios de Cultura
/
Técnicas de Cultura de Tecidos
/
Concentração de Íons de Hidrogênio
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2007
Tipo de documento:
Artigo
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