Regulation of E-cadherin promoter activity by LMO2 impact on the progression and metastasis of prostate cancer / 中国应用生理学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the abnormal expression of the proto-oncogene LMO2 affect the progression and metastasis mechanism of prostate cancer.</p><p><b>METHODS</b>A series of reporter gene expression vectors carrying different lengths and point mutations of E-cadherin promoter were constructed. These plasmids were separately co-transfected with LMO2 into Lncap cells and the luciferase activity was detected after 24 h.</p><p><b>RESULTS</b>The overexpression of LMO2 could significantly inhibit the activity of luciferase reporter gene of E-cadherin promoter about 50%. Truncated and point mutation study showed that this was mainly through E-box sites in the promoter region -204/-198.</p><p><b>CONCLUSION</b>The proto-oncogene LMO2 can affect the progression and metastasis mechanism of prostate cancer by transcriptional inhibition of E-cadherin.</p>