Altered expression of matrix metalloproteinases and tight junction proteins in rats following PEMF-induced BBB permeability change / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 197-202, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-235535
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of occludin, ZO-1, MMP-2, and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.</p><p><b>METHODS</b>Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n = 8). After exposure to PEMF at 0.5, 1, 3, 6, and 12 h, BBB permeability was measured by Evans-Blue extravasation. The expression of occludin, ZO-1, MMP-2, and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting. MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.</p><p><b>RESULTS</b>Compared with the sham group, PEMF exposure led to increased permeability of the BBB to EB, which was prolonged after exposure. BBB permeability became progressively more severe, and recovered at 6 h. The gene and protein expression of occludin and ZO-1 were significantly decreased, while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF. All levels of expression recovered 12 h following PEMF.</p><p><b>CONCLUSION</b>Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Barreira Hematoencefálica
/
Proteínas
/
Ratos Sprague-Dawley
/
Junções Íntimas
/
Metaloproteinases da Matriz
/
Campos Eletromagnéticos
/
Metabolismo
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2012
Tipo de documento:
Artigo
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