Gene expression changes in the pituitary gland of rats exposed to electromagnetic pulses / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 560-568, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-235596
ABSTRACT
<p><b>OBJECTIVE</b>We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).</p><p><b>METHODS</b>The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis. We then validated and further investigated the reduced expression of two tumorigenesis-related genes, Pten, and Jund, by assessing their mRNA and protein expression by quantitative real-time-PCR, western blotting, and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.</p><p><b>RESULTS</b>EMP exposure induced genome-wide gene expression changes in the rat pituitary gland. There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.</p><p><b>CONCLUSION</b>EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland. These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Hipófise
/
Neoplasias Hipofisárias
/
Adenoma
/
Regulação para Baixo
/
Proteínas Proto-Oncogênicas c-jun
/
Ratos Wistar
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
PTEN Fosfo-Hidrolase
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2011
Tipo de documento:
Artigo
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