Immune responses in wild-type mice against prion proteins induced using a DNA prime-protein boost strategy / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 523-529, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-235601
ABSTRACT
<p><b>OBJECTIVE</b>To break immune tolerance to prion (PrP) proteins using DNA vaccines.</p><p><b>METHODS</b>Four different human prion DNA vaccine candidates were constructed based on the pcDNA3.1 vector PrP-WT expressing wild-type PrP, Ubiq-PrP expressing PrP fused to ubiquitin, PrP-LII expressing PrP fused to the lysosomal integral membrane protein type II lysosome-targeting signal, and PrP-ER expressing PrP locating the ER. Using a prime-boost strategy, three-doses of DNA vaccine were injected intramuscularly into Balb/c mice, followed by two doses of PrP protein. Two weeks after the last immunization, sera and spleens were collected and PrP-specific humoral and cellular immune responses evaluated by ELISA and ELISPOT tests.</p><p><b>RESULTS</b>Higher levels of serum PrP antibodies were detected in mice vaccinated using the strategy of DNA priming followed by protein boosting. Of these, WT-PrP, Ubiq-PrP, and PrP-LII induced significantly higher humoral responses. ELISPOT tests showed markedly increased numbers of IFN-γ-secreting T cells in mice vaccinated using the strategy of DNA priming followed by protein boosting after stimulation with recombinant PrP23-90 and PrP23-231. PrP-ER induced the strongest T-cell response.</p><p><b>CONCLUSION</b>Prion vaccines can break tolerance to PrP proteins and induce PrP-specific humoral and cellular immune responses.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fragmentos de Peptídeos
/
Proteínas Recombinantes de Fusão
/
Príons
/
Proteínas Recombinantes
/
Ensaio de Imunoadsorção Enzimática
/
Células HeLa
/
Transfecção
/
Chlorocebus aethiops
/
Cricetulus
/
Interferon gama
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2011
Tipo de documento:
Artigo
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