Molecular identification of raw materials from lian qiao bai du wan / 药学学报
Acta Pharmaceutica Sinica
;
(12): 590-596, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-235623
ABSTRACT
Lian Qiao Bai Du Wan was used to study the identification of Chinese patent medicine by molecular marker technique. DNA was extracted through modified CTAB method. The psbA-trnH and rbcL sequences were gradient amplified, and PCR products were ligated with the pEASY-T5 vector and then transformed into Trans1-T1 cells, respectively. Clones were selected randomly and sequenced. All sequences were analyzed by BlastN and the neighbor-joining (NJ) phylogenetic tree was constructed by MEGA 4.0. The results showed that nine kinds of medicinal materials can be identified by psbA-trnH sequences, and six kinds of medicinal materials by rbcL sequences from Lian Qiao Bai Du Wan. Molecular marker technique can stably and accurately distinguish multi-origin medicinal materials in Chinese patent medicine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Plantas Medicinais
/
Ribulose-Bifosfato Carboxilase
/
Especificidade da Espécie
/
Medicamentos de Ervas Chinesas
/
Sequência de Bases
/
Análise por Conglomerados
/
Química
/
Cloroplastos
/
Reação em Cadeia da Polimerase
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2013
Tipo de documento:
Artigo
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