Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
Journal of Veterinary Science
;
: 99-101, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-23570
ABSTRACT
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Proteínas Recombinantes
/
Ensaio de Imunoadsorção Enzimática
/
Doenças dos Bovinos
/
Sensibilidade e Especificidade
/
Leptospira interrogans
/
Leptospirose
/
Lipoproteínas
/
Anticorpos Antibacterianos
/
Antígenos de Bactérias
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2012
Tipo de documento:
Artigo
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