Effect of salvianolic acid B on TNF-α induced cerebral microcirculatory changes in a micro-invasive mouse model / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 85-93, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-235776
ABSTRACT
<p><b>PURPOSE</b>To investigate the effects of salvianolic acid B (SAB) on tumor necrosis factor a (TNF-α) induced alterations of cerebral microcirculation with a bone-abrading model.</p><p><b>METHODS</b>The influences of craniotomy model and bone-abrading model on cerebral microcirculation were compared. The bone-abrading method was used to detect the effects of intracerebroventricular application of 40 μg/kg·bw TNF-α on cerebral venular leakage of fluorescein isothiocyanate (FITC)- albulmin and the rolling and adhesion of leukocytes on venules with fluorescence tracer rhodamine 6G. The therapeutical effects of SAB on TNF-α induced microcirculatory alteration were observed, with continuous intravenous injection of 5 mg/kg·h SAB starting at 20 min before or 20 min after TNF-α administration, respectively. The expressions of CD11b/CD18 and CD62L in leukocytes were measured with flow cytometry. Immunohistochemical staining was also used to detect E-selectin and ICAM-1 expression in endothelial cells.</p><p><b>RESULTS</b>Compared with craniotomy method, the bone-abrading method preserved a higher erythrocyte velocity in cerebral venules and more opening capillaries. TNF-α intervention only caused responses of vascular hyperpermeability and leukocyte rolling on venular walls, without leukocyte adhesion and other hemodynamic changes. Pre- or post-SAB treatment attenuated those responses and suppressed the enhanced expressions of CD11b/CD18 and CD62L in leukocytes and E-selectin and ICAM-1 in endothelial cells induced by TNF-α.</p><p><b>CONCLUSIONS</b>The pre- and post-applications of SAB during TNF-α stimulation could suppress adhesive molecular expression and subsequently attenuate the increase of cerebral vascular permeability and leukocyte rolling.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Valores de Referência
/
Benzofuranos
/
Velocidade do Fluxo Sanguíneo
/
Distribuição Aleatória
/
Circulação Cerebrovascular
/
Fator de Necrose Tumoral alfa
/
Molécula 1 de Adesão Intercelular
/
Selectina E
/
Craniotomia
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2016
Tipo de documento:
Artigo
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