The Role of CTGF in Osteosarcoma Progression / 대한골관절종양학회지
The Journal of the Korean Bone and Joint Tumor Society
;
: 1-6, 2014.
Artigo
em Coreano
| WPRIM
| ID: wpr-23599
ABSTRACT
PURPOSE:
To examine the expression of Connective Tissue Growth Factor (CTGF) in osteosarcoma and to evaluate its role in osteosarcoma invasion and proliferation. MATERIALS ANDMETHODS:
The mRNA expression of CTGF from 23 patient-derived osteosarcoma cell lines was examined, and the role of CTGF in cell invasion and proliferation was examined using siRNA transfection.RESULTS:
The over-expression of CTGF mRNA was observed in 17 cell lines (74%). CTGF-specific siRNA transfection into SaOS-2 and MG63 cell lines resulted in efficient knockdown of CTGF expression on Western blot analysis. siRNA transfected cells showed decreased migration on Matrigel invasion assay and decreased cell proliferation on WST-1 assay.CONCLUSION:
These results indicated that the CTGF expression may play an important role in osteosarcoma progression, and may be a therapeutic target of osteosarcoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Transfecção
/
Osteossarcoma
/
Linhagem Celular
/
Western Blotting
/
RNA Interferente Pequeno
/
Proliferação de Células
/
Fator de Crescimento do Tecido Conjuntivo
Idioma:
Coreano
Revista:
The Journal of the Korean Bone and Joint Tumor Society
Ano de publicação:
2014
Tipo de documento:
Artigo
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