Effect of human semaphorin-3F gene transient transfection on the proliferation of tongue carcinoma cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 726-728, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-235998
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of semaphorin-3F (SEMA-3F) gene transient transfection on the proliferation of Tca8113 tongue carcinoma cells.</p><p><b>METHODS</b>After construction of a full-length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by Lipofectamine 2000. The expression of SEMA-3F gene was detected by RT-PCR The differences of the expression in the transfected cell groups, empty vector groups and un-transfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium (MTT) enzymatic labeling technique. Cell cycle were assayed by flow cytometer (FCM).</p><p><b>RESULTS</b>The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased.</p><p><b>CONCLUSIONS</b>SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Neoplasias da Língua
/
Carcinoma de Células Escamosas
/
Transfecção
/
Ciclo Celular
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Vetores Genéticos
/
Genética
/
Proteínas de Membrana
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Artigo
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