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Simultaneous determination of 3 phenolic acids in Usnea by HPLC-ESI-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4884-4889, 2015.
Artigo em Chinês | WPRIM | ID: wpr-236027
ABSTRACT
A quick HPLC-ESI-MS/MS method was established for simultaneous determination of three chemical compositions in Usnea, including usnic acid, diffractaic acid, and ramalic acid. The separation was performed on a chromatographic column of Agilent ZORBAX SB-C, (4.6 mm x 250 mm, 5 µm), and the mobile phase was methanol (0.05% formic acid)-0.05% formic acid solution (4 mmol ammonium acetate), with an isocratic elution at a flow rate of 0.8 ml · min⁻¹. Multiple reaction monitoring scanning mode (MRM) was performed combined with the ion switching technology in positive and negative ion switching mode to apply for the quantitative determination. The calibration curves for the above three compounds were linear in corresponding injection amount. Their average recoveries were 95.0%-105.1%, with RSDs of 1.1%-5.2%. The method was simple, rapid, accurate with high repeatability, which could provide a reference for overcalling evaluation the quality of Usnea.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Química / Cromatografia Líquida de Alta Pressão / Usnea / Espectrometria de Massas por Ionização por Electrospray / Espectrometria de Massas em Tandem / Hidroxibenzoatos / Métodos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Química / Cromatografia Líquida de Alta Pressão / Usnea / Espectrometria de Massas por Ionização por Electrospray / Espectrometria de Massas em Tandem / Hidroxibenzoatos / Métodos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2015 Tipo de documento: Artigo