Effect of sodium tanshinone II A sulfonate on cardiac myocyte hypertrophy and its underlying mechanism / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 197-201, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-236267
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of sodium tanshinone II A sulfonate (STS) on the hypertrophy induced by angiotensin II (Ang II) in primary cultured neonatal rat cardiac myocytes.</p><p><b>METHODS</b>The effect of STS on cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-3,5-phenytetrazoliumromide (MTT) assay. As indexes for cardiocyte hypertrophy, cell size was determined by phase contrast microscopy and protein synthesis rate was measured by 3H-leucine incorporation. The proto-oncogene c-fos mRNA expression of cardiocytes was assessed using reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>STS could inhibit cardiocyte hypertrophy, increase the protein synthesis rate and enhance proto-oncogene c-fos mRNA expression in cardiocytes induced by Ang II (P<0.01), with an effect similar to that of Valsartan, the Ang II receptor antagonist.</p><p><b>CONCLUSION</b>STS can prevent the hypertrophy of cardiac myocytes induced by Ang II, which may be related to its inhibition of the expression of proto-oncogene c-fos mRNA.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Fenantrenos
/
Fatores de Tempo
/
Trítio
/
RNA Mensageiro
/
Angiotensina II
/
Sobrevivência Celular
/
Regulação da Expressão Gênica
/
Proteínas Proto-Oncogênicas c-fos
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese journal of integrative medicine
Ano de publicação:
2008
Tipo de documento:
Artigo
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