Efficacy comparison between transplanting microenvironmental induced and non-induced bone marrow mesenchymal stem cells in ischemic rat hearts / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 680-684, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-236426
ABSTRACT
<p><b>OBJECTIVE</b>To compare the efficacy of transplanting bone marrow mesenchymal stem cell (BMSC) or microenvironmental induced BMSC (iBMSC) into the ischemic myocardium of rats with myocardial infarction.</p><p><b>METHODS</b>iBMSC was defined as BMSC co-cultured with myocardial cells for 2 weeks. The stem cells or equal volume PBS were injected into ischemic border zone 1 wk after experimental infarction. Cardiac performance was evaluated at 1, 2, and 4 wk after cell transplantation by echocardiography and analyzed histologically at 4 wk after cell transplantations.</p><p><b>RESULTS</b>Compared with PBS group, both BMSC and iBMSC transplantations reduced infarct size. iBMSC enhanced the beneficial effects of BMSC on improving cardiac function (FS 28.5% +/- 4.3% in PBS, 29.0% +/- 2.0% in BMSC and 45.1% +/- 3.1% in iBMSC group at 4 weeks post transplantation, iBMSC group vs. PBS group P < 0.05, iBMSC group vs. BMSC group P < 0.05). Immunofluorescence microscopy results revealed co-localization of SPIO-labeled transplanted cells with cardiac markers for cardiomyocytes, indicating regeneration of damaged myocardium.</p><p><b>CONCLUSION</b>Our data suggest that iBMSC implantation is more effective on improving cardiac function than BMSC implantation in this model. iBMSC might serve as a new promising therapeutic cell source for regenerating ischemic myocardium in patients with post-infarction heart failure.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cirurgia Geral
/
Diferenciação Celular
/
Células Cultivadas
/
Transplante de Medula Óssea
/
Ratos Sprague-Dawley
/
Condicionamento Pré-Transplante
/
Transplante de Células-Tronco Mesenquimais
/
Infarto do Miocárdio
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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