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Expression of MDR1 and GST-pi in osteosarcoma and soft tissue sarcoma and their correlation with chemotherapy resistance / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 445-448, 2006.
Artigo em Chinês | WPRIM | ID: wpr-236920
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of multidrug resistance gene 1 ( MDR1), glutathione-S-transferases-pi (GST-pi) in osteosarcoma and soft tissue sarcoma tissues from 34 patients and their correlation with chemotherapy resistance.</p><p><b>METHODS</b>MDR1 and GST-pi expressions were analyzed by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) and flow cytometry (FCM) at mRNA and protein levels, respectively. Chemotherapy sensitivity on adriamycin, cisplatinum, fluorouracil, mitomycin C, dacarbazine, vincristine, methotrexate in tumor tissues were detected by MTT assay.</p><p><b>RESULTS</b>The nonsensitive rates on adriamycin, cisplatinum, fluorouracil, mitomycin C, dacarbazine, vincristine, methotrexate in tumor tissues were 41.18%, 17.7%, 47.1%, 50.0%, 76.5%, 61.8% and 52.9%, respectively. The expression of P-glycoprotein (P-gp) and GST-pi in tumor tissues was 1.54 and 2.58 (relative fluorescence intensity). Chi2 analysis showed that there was a positive correlation between P-gp expression and drug resistance on ADM, GST-pi expression and resistance on ADM, DDP and MMC (P < 0.05). There was not seen obvious correlation between expression of MDR1, GST-pi and age, gender, pathological type, tumor size in osteosarcoma and soft tissue sarcoma patients (P > 0.05). The expression of GST-pi was increased in patients receiving preoperative chemotherapy. The rate of postoperative recurrence was higher in patients with higher GST-pi expression level than those with lower GST-pi expression level before operation (P < 0.05).</p><p><b>CONCLUSION</b>Individual differences exist in chemotherapy sensitivity and expression of MDR1 and GST-pi in osteosarcoma and soft tissue sarcomas patients. Chemotherapy can induce up-regulation of GST-pi protein expression. Primary high expression of GST-pi is the main mechanism of resistance of osteosarcoma and soft tissue sarcomas to chemotherapy and is related to poor prognosis.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Prognóstico / Sarcoma / Neoplasias Ósseas / RNA Mensageiro / Protocolos de Quimioterapia Combinada Antineoplásica / Doxorrubicina / Osteossarcoma / Seguimentos / Cisplatino / Membro 1 da Subfamília B de Cassetes de Ligação de ATP Tipo de estudo: Estudo observacional / Estudo prognóstico Limite: Adolescente / Adulto / Idoso / Criança / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Prognóstico / Sarcoma / Neoplasias Ósseas / RNA Mensageiro / Protocolos de Quimioterapia Combinada Antineoplásica / Doxorrubicina / Osteossarcoma / Seguimentos / Cisplatino / Membro 1 da Subfamília B de Cassetes de Ligação de ATP Tipo de estudo: Estudo observacional / Estudo prognóstico Limite: Adolescente / Adulto / Idoso / Criança / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2006 Tipo de documento: Artigo