Polyadenylation signal-deficient retroviruses transformation of human gastric epithelial GES-1 cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 337-341, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-236971
ABSTRACT
<p><b>OBJECTIVE</b>To verify if mutated polyadenylation signal retroviruses can produce viral-host readthrough transcripts (Rth) and have the ability to transform human gastric epithelial GES-1 cells, and to discuss the new functions of retroviruses in gastric cancer related gene research.</p><p><b>METHODS</b>The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells. The GES-1 cells were infected by the viruses and selected by G418. Viral-host readthrough RNAs were checked by Northern blot. The cell growth and soft agar assay were run to test the transformed cells.</p><p><b>RESULTS</b>polyadenylation signal-deficient retroviruses could be packaged by PA317 packaging cells. The viruses had the ability to infect GES-1 cells. Northern blot analysis of viral RNA from infected pools and individual G418-resistant clones demonstrated that mutation of consensus LTR polyadenylation signals generated Rth viral RNA in the infected GES-1 cells. Phenotypic analysis results showed that the GES-1 cells infected with plyadenylation signal mutant viruses tended to grow in a cluster manner. Pools of PA317 cells infected with mutant viruses were able to form colonies in soft agar with a higher efficiency than control or uninfected cells.</p><p><b>CONCLUSION</b>Host readthrough transcripts generated by polyadenylation signal mutant viruses may contribute to transformation GES-1 cell phenotypes. The mutant vectors and the method described in the present work may be useful as tools to trap and identify genes involved in retroviral insertion mediated cell transformation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Retroviridae
/
Estômago
/
Virologia
/
RNA Viral
/
Linhagem Celular
/
Transformação Celular Neoplásica
/
Mutagênese Sítio-Dirigida
/
Sequências Repetidas Terminais
/
Biologia Celular
/
Sinais de Poliadenilação na Ponta 3' do RNA
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2006
Tipo de documento:
Artigo
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