Study on rhG-CSF modified with polyethylene glycol / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 965-969, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-237042
ABSTRACT
Monomethoxy Polyethylene Glycol(mPEG20000) was activated by N-hydroxysuccinimede and analyzed by infrared spectrum and hydrolysis kinetics. In order to propose the optimized reaction conditions of mono-PEGylated rhG-CSF, orthogonal design of the experiment was investigated. Ion exchange chromatography was used to separate and purify PEGylated rhG-CSF from unPEGylated rhG-CSF. The purity of mono-PEGylated rhG-CSF was analyzed by high performance liquid chromatography (HPLC) to be 97%.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polietilenoglicóis
/
Propriedades de Superfície
/
Proteínas Recombinantes
/
Química
/
Fator Estimulador de Colônias de Granulócitos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS