Genetically modified industrial brewing yeast with high-glutathione and low-diacetyl production / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 942-946, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-237046
ABSTRACT
Recombinant plasmid pICG was constructed by replacing the internal fragment of a-acetohydroxyacid synthase (AHAS) gene (ILV2) with a copy of gamma-glutamylcysteine synthetase gene (GSH1) and copper chelatin gene (CUP1) from the industrial brewing yeast strain YSF31. YSF31 was transformed with plasmid pICG linearized by Kpn I and Pst I. A recombinant strain with high-glutathione and low-diacetyl production was selected. The results of fermentation in 100-L bioreactor showed that the lagering time of beer produced for recombinant strain T2 was shortened by 3 days and the shelf life of the beer was prolonged about 50%. It may be more acceptable for the commercial application, as it does not contain foreign DNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Acetolactato Sintase
/
Saccharomyces cerevisiae
/
Cerveja
/
Regulação Fúngica da Expressão Gênica
/
Clonagem Molecular
/
Organismos Geneticamente Modificados
/
Proteínas de Saccharomyces cerevisiae
/
Diacetil
/
Fermentação
/
Glutamato-Cisteína Ligase
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Artigo
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