The expression of VP19 gene from prawn white spot syndrome virus in silkworm, Bombyx mori using host range-expanded HyNPV / 生物工程学报
Chinese Journal of Biotechnology
; (12): 837-839, 2005.
Article
em Zh
| WPRIM
| ID: wpr-237064
Biblioteca responsável:
WPRO
ABSTRACT
Prawn white spot syndrome is caused by the pathogen prawn white spot syndrome virus (WSSV). VP19 is a vesicle membrane protein of WSSV. HyNPV (Hybrid of AcNPV and BmNPV) constructed by the recombination of BmNPV and AcNPV is a new hybrid virus having both of their advantages. The recombinant transfer vector pBlueBicHisC-vp19 and recombinant baculovirus HyNPV-VP19 were constructed on the basis of the successful cloning of VP19. Newly-molted silkworms Bombyx mori of fifth instar were inoculated by the recombinant virus. SDS-PAGE and Western blotting analysis showed a specific band, about 21kD, which was consistent with the expectation suggesting that the WSSV-VP19 gene was successfully expressed in silkworm bodies.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Bombyx
/
Virologia
/
Replicação Viral
/
Proteínas Recombinantes de Fusão
/
Proteínas do Envelope Viral
/
Baculoviridae
/
Penaeidae
/
Vírus da Síndrome da Mancha Branca 1
/
Vetores Genéticos
/
Genética
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Article